Article
MUTZ-3-derived dendritic cells as an in vitro alternative model to CD34+ progenitor-derived dendritic cells for testing of chemical sensitizers.
Toxicology in vitro : an international journal published in association with BIBRA - 1 Dec 2009
Nelissen Inge, Selderslaghs Ingrid, Heuvel Rosette Van Den, Witters Hilda, Verheyen Geert R, Schoeters Greet
Abstract excerpt
The cytokine-dependent CD34(+) human acute myeloid leukaemia cell line MUTZ-3 was used to generate immature dendritic-like cells (MUTZ-3 DC) and their validity as an alternative to primary CD34(+) progenitor-derived DC (CD34-DC) for testing chemical-induced sensitization was assessed. Expression levels of the DC maturation markers HLA-DR, CD86, CD83 and CD11c were studied using flow cytometry after 24 and 48 h...
Topics
- Antigens, CD34
- Cell Line, Tumor
- Cytokines
- Dendritic Cells
- Hematopoietic Stem Cells
- Humans
- Nickel
- Phenotype