Simplified method to generate large quantities of dendritic cells suitable for clinical applications.

doi:10.3109/08820130009060870

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Simplified method to generate large quantities of dendritic cells suitable for clinical applications.

Immunological investigations - 1 Aug 2000

Goxe B, Latour N, Chokri M, Abastado J P, Salcedo M

Abstract excerpt

The present study describes the optimization of an in vitro culture method for generating large amounts of dendritic cells (DC) in serum-free conditions from leukapheresis containing a mixed population of peripheral blood mononuclear cells (PBMC) which are cultured in the presence of GM-CSF and IL-13. Initial comparisons between the generation of DC from bulk and monocyte-enriched leukapheresis products showed...

Topics

Cell Communication
Cell Differentiation
Cell Separation
Culture Media
Culture Media, Serum-Free
Culture Techniques
Cytokines
Dendritic Cells
Humans
Leukapheresis
Leukocytes, Mononuclear
Lymphocytes
Monocytes
Phenotype

Source

Open in PubMed

Identifiers

PMID: 10933613
DOI: 10.3109/08820130009060870

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics