Generation of specificity-variant antibodies by alteration of carbohydrate in light chain of human monoclonal antibodies.

doi:10.1016/0006-291x(92)92246-t

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Article

Generation of specificity-variant antibodies by alteration of carbohydrate in light chain of human monoclonal antibodies.

Biochemical and biophysical research communications - 15 Dec 1992

Tachibana H, Shirahata S, Murakami H

Abstract excerpt

A hybridoma line, C5TN, produced human monoclonal antibody of which light chain had N-linked carbohydrate chain within the variable region. Some molecular-weight variants of light chain of the antibody were produced by C5TN variants resistant to cytotoxic effect of concanavalin A. The variant antibodies significantly altered the original cross-reactivity with antigens or lost the ability of antigen binding. The...

Topics

Antibodies, Monoclonal
Antibody Specificity
Antigen-Antibody Complex
Blotting, Western
Carbohydrates
Cell Line
Cell Survival
Clone Cells
Concanavalin A
Enzyme-Linked Immunosorbent Assay

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Variation

Glycoside Hydrolases

Glycosylation

Humans

Hybridomas

Immunoglobulin Light Chains

Oligosaccharides

Source

Open in PubMed

Identifiers

PMID: 1472033
DOI: 10.1016/0006-291x(92)92246-t

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1472033-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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