Article
[Preparation and characterization of a mouse anti-human CD40 mutant monoclonal antibody].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Oct 2009
Zheng Shu-Dan, Ma Hong-Bing, Gao Chao, Whang Jia-Min, Sun Jing, Luo Xian-Fu, Zhang Xue-Guang
Abstract excerpt
AIM: To prepare and characterize a mouse anti-human CD40 mutant monoclonal mAb. METHODS: Female BALB/c mice of 6-8 weeks old were immunized with CD40 mutant transfectant (L929-CD40mu) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells.The hybridoma cells were screened with CD40 mutant transfectant (L929-CD40mu) by FCM. Fast-strip analysis was performed to identify Ig subclass of this...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
