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Article

Hybridoma-free generation of monoclonal antibodies.

Proceedings of the National Academy of Sciences of the United States of America - 6 Jan 2004

Pasqualini Renata, Arap Wadih

Abstract excerpt

Production of monoclonal antibodies requires immortalization of splenocytes by somatic fusion to a myeloma cell line partner (hybridomas). Although hybridomas can be immortal, they may depend on a feeder cell layer and may be genetically unstable. Since the inception of hybridoma technology, efforts to improve efficiency and stability of monoclonal antibody-producing cell lines have not brought about substantial...

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Share to Antibodies, Monoclonal
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 14688405
DOI: 10.1073/pnas.0305834101
PMCID: PMC314172

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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