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Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies

27 Feb 2015

Abstract excerpt

The human hybridoma technique offers an important approach for isolation of human monoclonal antibodies. A diversity of approaches can be used with varying success. Recent technical advances in expanding the starting number of human antigen-specific B cells, improving fusion efficiency, and isolating new myeloma partners and new cell cloning methods have enabled the development of protocols that make the...

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PMID: 26104564
DOI: 10.1128/microbiolspec.aid-0027-2014

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