Article
Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavage.
Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 2009
Li Xiaochun, Wang Boyuan, Feng Lihui, Kang Hui, Qi Yang, Wang Jiawei, Shi Yigong
Abstract excerpt
Regulated intramembrane proteolysis (RIP) by the Site-2 protease (S2P) results in the release of a transmembrane signaling protein. Curiously, however, S2P cleavage must be preceded by the action of the Site-1 protease (S1P). To decipher the underlying mechanism, we reconstituted sequential, in vitro cleavages of the Escherichia coli transmembrane protein RseA by DegS (S1P) and RseP (S2P). After DegS cleavage,...
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