Article
Structure-function studies of interferon-alpha based on random mutagenesis and expression in vitro.
Genetic analysis, techniques and applications - 1 May 1990
Tymms M J, McInnes B, Alin P, Linnane A W, Cheetham B F
Abstract excerpt
An efficient procedure for random chemical mutagenesis was used to create analogs of human interferon (IFN)-alpha 4. Unique restriction enzyme sites were introduced into the human IFN-alpha 4 gene to enable cassetting of the gene for localized random mutagenesis. Single-stranded IFN-alpha 4 DNA was treated with nitrous acid, followed by second-strand synthesis using reverse transcriptase. A 72 base pair cassette...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Deoxyribonucleases
- Electrophoresis
- Gene Expression
- Humans
- Interferon Type I
- Molecular Sequence Data
- Mutation
- Polymorphism, Restriction Fragment Length