Article
Efficient in vitro expression of interferon alpha analogs using SP6 polymerase and rabbit reticulocyte lysate.
Gene analysis techniques - 1 Jan 2000
Tymms M J, McInnes B
Abstract excerpt
We have investigated the use of in vitro expression as a quick and convenient means of screening large numbers of interferon (IFN) analogs generated using in vitro mutagenesis. The IFN-alpha 1 mRNA generated from DNA template using SP6 RNA polymerase is efficiently translated in rabbit reticulocyte lysate (RRL). The antiviral specific activity of this RRL-synthesized IFN-alpha l is equivalent to the...
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