Efficient in vitro expression of interferon alpha analogs using SP6 polymerase and rabbit reticulocyte lysate.

doi:10.1016/0735-0651(88)90021-0

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Article

Efficient in vitro expression of interferon alpha analogs using SP6 polymerase and rabbit reticulocyte lysate.

Gene analysis techniques - 1 Jan 2000

Tymms M J, McInnes B

Abstract excerpt

We have investigated the use of in vitro expression as a quick and convenient means of screening large numbers of interferon (IFN) analogs generated using in vitro mutagenesis. The IFN-alpha 1 mRNA generated from DNA template using SP6 RNA polymerase is efficiently translated in rabbit reticulocyte lysate (RRL). The antiviral specific activity of this RRL-synthesized IFN-alpha l is equivalent to the...

Topics

Animals
Biological Assay
Cell-Free System
Cloning, Molecular
DNA-Directed RNA Polymerases
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression Regulation
Interferon Type I
Methionine
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Biosynthesis

RNA, Messenger

Rabbits

Reticulocytes

Saccharomyces cerevisiae

Transcription, Genetic

Source

Open in PubMed

Identifiers

PMID: 3056802
DOI: 10.1016/0735-0651(88)90021-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-3056802-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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