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Article

A point mutation of human interferon gamma abolishes receptor recognition.

Protein engineering - 1 Apr 1992

Lunn C A, Fossetta J, Dalgarno D, Murgolo N, Windsor W, Zavodny P J, Narula S K, Lundell D

Abstract excerpt

We identified a single amino acid mutation that abolished the bioactivity of human IFN gamma. The mutation was identified by screening a mutagenized IFN gamma expression library for molecules with altered biological activity. The mutant protein was expressed at high levels in Escherichia coli, and remained soluble upon purification. However, the protein was completely inactive in all IFN gamma assays...

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Identifiers

PMID: 1409546
DOI: 10.1093/protein/5.3.253

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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