Article
A point mutation of human interferon gamma abolishes receptor recognition.
Protein engineering - 1 Apr 1992
Lunn C A, Fossetta J, Dalgarno D, Murgolo N, Windsor W, Zavodny P J, Narula S K, Lundell D
Abstract excerpt
We identified a single amino acid mutation that abolished the bioactivity of human IFN gamma. The mutation was identified by screening a mutagenized IFN gamma expression library for molecules with altered biological activity. The mutant protein was expressed at high levels in Escherichia coli, an...
Topics
- Amino Acid Sequence
- Circular Dichroism
- Cytopathogenic Effect, Viral
- Escherichia coli
- Humans
- Interferon-gamma
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Protein Engineering
- Receptors, Interferon
- Recombinant Proteins