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Article

Improvement of extracellular production of a thermophilic subtilase expressed in Escherichia coli by random mutagenesis of its N-terminal propeptide.

Applied microbiology and biotechnology - 1 Feb 2010

Fang Nan, Zhong Chuan-Qi, Liang Xiaoliang, Tang Xiao-Feng, Tang Bing

Abstract excerpt

Limited secretion capacity remains a drawback of using Escherichia coli as the host for the production of recombinant proteins. In this report, random mutagenesis was performed within the N-terminal propeptide of thermostable WF146 protease, a subtilase from thermophilic Bacillus sp. WF146, generating a variant named WBM(MT) with improved capacity for extracellular production when expressed in E. coli. Two...

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PMID: 19697018
DOI: 10.1007/s00253-009-2183-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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