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Article

Improvement in organic solvent resistance and activity of metalloprotease by directed evolution.

Journal of biotechnology - 10 Feb 2020

Zhu Fucheng, He Bingfang, Gu Fangli, Deng Hui, Chen Chunwu, Wang Weiyun, Chen Naifu

Abstract excerpt

Improving enzyme stability in the presence of organic solvent is crucial for non-aqueous catalysis. In this study, directed evolution was applied to improve the tolerance of metalloprotease PT121 towards organic solvent. In presence of acetonitrile and acetone, three mutants (T46Y, H224 F, and H224Y) of PT121 showed excellent solvent stability, which increased their half-lives by 1.2-3.5-fold as compared to the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 31870685
DOI: 10.1016/j.jbiotec.2019.12.014

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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