Article
Importance of C-Terminal Extension in Thermophilic 1,4-α-Glucan Branching Enzyme from Geobacillus thermoglucosidans STB02.
Applied biochemistry and biotechnology - 1 Mar 2020
Ban Xiaofeng, Li Caiming, Zhang Yuzhu, Gu Zhengbiao, Cheng Li, Hong Yan, Li Zhaofeng
Abstract excerpt
By sequence comparison, the majority of 1,4-α-glucan-branching enzymes (GBEs) consist of an N-terminal carbohydrate-binding domain, a TIM-barrel catalytic domain, and a C-terminal all-beta domain. Among these structures, the GBE from Geobacillus thermoglucosidans STB02 uniquely has a highly charged 26-amino-acid C-terminal extension, whose functional roles are the least understood. In this research, the...
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