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Enhancement of keratinolytic activity of a thermophilic subtilase by improving its autolysis resistance and thermostability under reducing conditions.

Applied microbiology and biotechnology - 1 Jul 2010

Liang Xiaoliang, Bian Yan, Tang Xiao-Feng, Xiao Gengfu, Tang Bing

Abstract excerpt

WF146 protease, a thermophilic subtilase from thermophile Bacillus sp. WF146, suffers excessive autolysis in the presence of reducing agents. In this report, two autolytic sites of WF146 protease were modified by site-directed mutagenesis. The introduction of prolines into the autolytic sites increased the autolysis resistance of the enzyme under reducing conditions. The double mutant N63P/A66P displayed a...

Topics

Animals
Bacillus
Bacterial Proteins
Enzyme Stability
Feathers
Hot Temperature
Hydrolysis
Kinetics
Mutagenesis, Site-Directed
Mutation
Subtilisins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 20306186
DOI: 10.1007/s00253-010-2534-2

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