Article
Mechanism of polymerase collision release from sliding clamps on the lagging strand.
The EMBO journal - 7 Oct 2009
Georgescu Roxana E, Kurth Isabel, Yao Nina Y, Stewart Jelena, Yurieva Olga, O'Donnell Mike
Abstract excerpt
Replicative polymerases are tethered to DNA by sliding clamps for processive DNA synthesis. Despite attachment to a sliding clamp, the polymerase on the lagging strand must cycle on and off DNA for each Okazaki fragment. In the 'collision release' model, the lagging strand polymerase collides with the 5' terminus of an earlier completed fragment, which triggers it to release from DNA and from the clamp. This...
Topics
- Binding Sites
- DNA Polymerase III
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- Protein Structure, Tertiary