Article
T cell receptor (TcR) beta chain transgenic mice: studies on allelic exclusion and on the TcR+ gamma/delta population.
European journal of immunology - 1 Feb 1990
Pircher H, Ohashi P, Miescher G, Lang R, Zikopoulos A, Bürki K, Mak T W, MacDonald H R, Hengartner H
Abstract excerpt
To study allelic exclusion of TcR genes we analyzed two types (I and II) of TcR beta transgenic mice. T cells derived from both types of mice contained similar amounts of transgenic RNA transcripts; however, surface expression of the transgenic beta chain was drastically reduced in type II compared to type I. In type I transgenic mice, productive rearrangements and expression of endogenous TcR beta genes were...
Topics
- Alleles
- Animals
- Antigens, Differentiation, T-Lymphocyte
- Antigens, Surface
- Blotting, Northern
- CD3 Complex
- Flow Cytometry
- Gene Expression Regulation
- Gene Rearrangement, T-Lymphocyte
- Hybridomas