Article
Essential role of the pre-T cell receptor in allelic exclusion of the T cell receptor beta locus.
Immunity - 1 Nov 1997
Aifantis I, Buer J, von Boehmer H, Azogui O
Abstract excerpt
Following the recent realization that TCR beta transgenes can severely inhibit the rearrangement of endogenous Vbeta gene segments in the absence of pre-TCR alpha (pT alpha) chains, we tested whether the pre-TCR has an essential role in TCR beta allelic exclusion under more physiological conditions by analyzing TCR rearrangement in immature thymocytes by single-cell PCR. Our results in pT alpha+ mice are...
Topics
- Alleles
- Animals
- Cell Differentiation
- Gene Expression Regulation, Developmental
- Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
- Genes, T-Cell Receptor alpha
- Genes, T-Cell Receptor beta
- Mice
- Mice, Knockout
- Mice, Transgenic
- Polymerase Chain Reaction
- Protein Processing, Post-Translational
- Receptors, Antigen, T-Cell, alpha-beta
- T-Lymphocytes
- Thymus Gland