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Article

Bacterial Sec protein transport is rate-limited by precursor length: a single turnover study.

Molecular biology of the cell - 1 Oct 2009

Liang Fu-Cheng, Bageshwar Umesh K, Musser Siegfried M

Abstract excerpt

An in vitro real-time single turnover assay for the Escherichia coli Sec transport system was developed based on fluorescence dequenching. This assay corrects for the fluorescence quenching that occurs when fluorescent precursor proteins are transported into the lumen of inverted membrane vesicles. We found that 1) the kinetics were well fit by a single exponential, even when the ATP concentration was...

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Identifiers

PMID: 19656854
DOI: 10.1091/mbc.e09-01-0075
PMCID: PMC2754939

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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