Article
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 1 Aug 2009
Andreoni Alessandra, Bondani Maria, Nardo Luca
Abstract excerpt
By tens-of-picosecond resolved fluorescence detection we study Förster resonance energy transfer between a donor and a black-hole-quencher acceptor bound at the 5'- and 3'-positions of a probe-oligonucleotide matching an allelic region of a class II antigen of the human leukocyte antigen system, namely the region between codons 52 and 57 of the HLA DQB1 0201 allele. This dual-labelled probe is annealed with...
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