Article
A new ultrasensitive way to circumvent PCR-based allele distinction: direct probing of unamplified genomic DNA by solution-phase hybridization using two-color fluorescence cross-correlation spectroscopy.
Experimental and molecular pathology - 1 Jun 2005
Földes-Papp Zeno, Kinjo Masataka, Tamura Mamoru, Birch-Hirschfeld Eckhard, Demel Ulrike, Tilz Gernot P
Abstract excerpt
Single-molecule fluorescence methods enable a new class of nucleic acid assays to be performed that are not possible with PCR-based methods. In this basic study, the methylene tetrahydrofolate reductase (MTHFR)-genotypes (normal, homozygous mutated, as well as heterozygous mutated) were directly detected for the first time onto unamplified double-stranded genomic DNA in solution down to femtomolar allele...
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