Article
Quantitative genotyping of single nucleotide polymorphism by single-molecule multi-color fluorescence resonance energy transfer.
Chemical communications (Cambridge, England) - 7 Oct 2011
Koh Hye Ran, Han Kyu Young, Jung Jiwon, Kim Seong Keun
Abstract excerpt
We developed a new single nucleotide polymorphism (SNP) genotyping method based on single-molecule multi-color fluorescence resonance energy transfer (FRET). We demonstrated that this new method uses less than 1 fmol of sample and is also highly quantitative with a detection level of 1% or lower in the minor allele fraction.
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