Article
SNP detection in mRNA in living cells using allele specific FRET probes.
PloS one - 1 Jan 2013
Dahan Liya, Huang Lingyan, Kedmi Ranit, Behlke Mark A, Peer Dan
Abstract excerpt
Live mRNA detection allows real time monitoring of specific transcripts and genetic alterations. The main challenge of live genetic detection is overcoming the high background generated by unbound probes and reaching high level of specificity with minimal off target effects. The use of Fluorescence Resonance Energy Transfer (FRET) probes allows differentiation between bound and unbound probes thus decreasing...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
