Article
Typing of a polymorphic human gene conferring susceptibility to insulin-dependent diabetes mellitus by picosecond-resolved FRET on non-purified/non-amplified genomic DNA.
DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Jan 2012
Nardo Luca, Tosi Giovanna, Bondani Maria, Accolla Roberto S, Andreoni Alessandra
Abstract excerpt
This work concerns the identification of the alleles of the polymorphic DQB1 gene of the human leukocyte antigen system, conferring susceptibility to the development of insulin-dependent diabetes mellitus (IDDM) in non-PCR amplified DNA samples and, more importantly, in crude cell extracts. Our method is based on the time-resolved analysis of a Förster energy-transfer mechanism that occurs in a dual-labelled...
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