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Article

Engineering of family-5 glycoside hydrolase (Cel5A) from an uncultured bacterium for efficient hydrolysis of cellulosic substrates.

PloS one - 1 Jan 2013

Telke Amar A, Zhuang Ningning, Ghatge Sunil S, Lee Sook-Hee, Ali Shah Asad, Khan Haji, Um Youngsoon, Shin Hyun-Dong, Chung Young Ryun, Lee Kon Ho, Kim Seon-Won

Abstract excerpt

Cel5A, an endoglucanase, was derived from the metagenomic library of vermicompost. The deduced amino acid sequence of Cel5A shows high sequence homology with family-5 glycoside hydrolases, which contain a single catalytic domain but no distinct cellulose-binding domain. Random mutagenesis and cellulose-binding module (CBM) fusion approaches were successfully applied to obtain properties required for cellulose...

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PMID: 23785445
DOI: 10.1371/journal.pone.0065727
PMCID: PMC3681849

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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