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Article

Highly thermostable fungal cellobiohydrolase I (Cel7A) engineered using predictive methods.

Protein engineering, design & selection : PEDS - 1 Dec 2012

Komor Russell S, Romero Philip A, Xie Catherine B, Arnold Frances H

Abstract excerpt

Building on our previous efforts to generate thermostable chimeric fungal cellobiohydrolase I (CBH I, also known as Cel7A) cellulases by structure-guided recombination, we used FoldX and a 'consensus' sequence approach to identify individual mutations present in the five homologous parent CBH I enzymes which further stabilize the chimeras. Using the FoldX force field, we calculated the effect on ΔG(Folding) of...

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PMID: 22961332
DOI: 10.1093/protein/gzs058

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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