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Article

Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability.

Protein engineering, design & selection : PEDS - 1 Nov 2007

Ni Jinfeng, Takehara Motomi, Miyazawa Mitsuhiro, Watanabe Hirofumi

Abstract excerpt

There have been two major problems preventing applications of termite cellulases; one was difficulty for their hetelologous overexpression, and another is their low thermostability. We previously achieved adaptation of termite cellulase genes to an overexpression system of Escherichia coli by family shuffling of four orthologous cDNAs (Biosci. Biotechnol. Biochem., 2005; 69: 1711-1720). Using the adapted mutant...

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PMID: 17971397
DOI: 10.1093/protein/gzm052

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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