Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library.

doi:10.1093/protein/gzs072

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Article

Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library.

Protein engineering, design & selection : PEDS - 1 Jan 2013

Ito Y, Ikeuchi A, Imamura C

Abstract excerpt

We aimed at constructing thermostable cellulase variants of cellobiohydrolase II, derived from the mesophilic fungus Phanerochaete chrysosporium, by using an advanced evolutionary molecular engineering method. By aligning the amino acid sequences of the catalytic domains of five thermophilic fung...

Topics

Amino Acid Sequence
Cellulase
Directed Molecular Evolution
Enzyme Stability
Gene Library
Models, Molecular
Molecular Sequence Data
Mutation
Phanerochaete
Protein Conformation
Temperature

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