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Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library.

Protein engineering, design & selection : PEDS - 1 Jan 2013

Ito Y, Ikeuchi A, Imamura C

Abstract excerpt

We aimed at constructing thermostable cellulase variants of cellobiohydrolase II, derived from the mesophilic fungus Phanerochaete chrysosporium, by using an advanced evolutionary molecular engineering method. By aligning the amino acid sequences of the catalytic domains of five thermophilic fungal CBH2 and PcCBH2 proteins, we identified 45 positions where the PcCBH2 genes differ from the consensus sequence of...

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PMID: 23091162
DOI: 10.1093/protein/gzs072

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