Article
Efficient transformation of cam2, a behavioral mutant of Paramecium tetraurelia, with the calmodulin gene.
Proceedings of the National Academy of Sciences of the United States of America - 1 Dec 1991
Kanabrocki J A, Saimi Y, Preston R R, Haynes W J, Kung C
Abstract excerpt
An Ile-136----Thr substitution in calmodulin reduces the Ca(2+)-dependent K+ currents of cam2, a behavioral mutant of Paramecium tetraurelia, and renders it overly susceptible to BaCl2. DNA fragments carrying the wild-type CAM gene injected into cam2 macronuclei reverted these phenotypes in the c...
Topics
- Amino Acid Sequence
- Animals
- Barium
- Barium Compounds
- Base Sequence
- Calmodulin
- Chlorides
- DNA
- Membrane Potentials
- Microinjections
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Paramecium
- Potassium Channels
- Restriction Mapping
- Transformation, Genetic