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Article

Transformation of Paramecium tetraurelia by electroporation or particle bombardment.

The Journal of eukaryotic microbiology - 1 Jan 2000

Boileau A J, Kissmehl R, Kanabrocki J A, Saimi Y

Abstract excerpt

Methods for mass transformation of Paramecium tetraurelia were established using plasmids bearing neomycin-resistance or calmodulin gene fragments. Phenotypic and molecular analyses showed that, although variable, up to 5% transformation can be achieved by electroporation. Concentrations of dival...

Topics

Animals
Calmodulin
Electroporation
Kanamycin Kinase
Mutagenesis
Paramecium tetraurelia
Phenotype
Transformation, Genetic

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 10188261
DOI: 10.1111/j.1550-7408.1999.tb04584.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026. Prepared record publication-10188261-2026-08-31.1. Manifest publication-graph-cohort-2026-08-31.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 31 Aug 2026.

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