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Article

Transformation of Paramecium tetraurelia by electroporation or particle bombardment.

The Journal of eukaryotic microbiology - 1 Jan 2000

Boileau A J, Kissmehl R, Kanabrocki J A, Saimi Y

Abstract excerpt

Methods for mass transformation of Paramecium tetraurelia were established using plasmids bearing neomycin-resistance or calmodulin gene fragments. Phenotypic and molecular analyses showed that, although variable, up to 5% transformation can be achieved by electroporation. Concentrations of divalent cations Ca2+ and Mg2+ in the electroporation medium were crucial for efficient transformation. Strong...

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PMID: 10188261
DOI: 10.1111/j.1550-7408.1999.tb04584.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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