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Article

The cloning and molecular analysis of pawn-B in Paramecium tetraurelia.

Genetics - 1 Jul 2000

Haynes W J, Ling K Y, Preston R R, Saimi Y, Kung C

Abstract excerpt

Pawn mutants of Paramecium tetraurelia lack a depolarization-activated Ca(2+) current and do not swim backward. Using the method of microinjection and sorting a genomic library, we have cloned a DNA fragment that complements pawn-B (pwB/pwB). The minimal complementing fragment is a 798-bp open reading frame (ORF) that restores the Ca(2+) current and the backward swimming when expressed. This ORF contains a 29-bp...

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Identifiers

PMID: 10880473
DOI: 10.1093/genetics/155.3.1105
PMCID: PMC1461171

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