Article
Inactivation of the p53 gene expression by a splice donor site mutation in a human T-cell leukemia cell line.
Leukemia - 1 Oct 1991
Soudon J, Caron de Fromentel C, Bernard O, Larsen C J
Abstract excerpt
An abnormally sized 3.5 kb p53 transcript was detected in the KE-37R human leukemic T-cell line in which no p53 protein could be detected by immunoprecipitation. S1 nuclease protection experiments and sequencing analysis indicated conservation of the entire intron 4 (755 bp) in the 3.5 kb transcript and the presence of a G to A substitution in the last exonic nucleotide of the splice donor site. These data...
Topics
- Animals
- Base Sequence
- DNA
- DNA, Recombinant
- Gene Expression Regulation, Leukemic
- Genes, p53
- Humans
- Introns
- Leukemia, T-Cell
- Mice
- Molecular Sequence Data
- Mutation