Article
Enhanced expression of engineered ACA-less beta-1, 6-N-acetylglucosaminidase (dispersin B) in Escherichia coli.
Journal of industrial microbiology & biotechnology - 1 Oct 2009
Yakandawala Nandadeva, Gawande Purushottam V, LoVetri Karen, Romeo Tony, Kaplan Jeffrey B, Madhyastha Srinivasa
Abstract excerpt
beta-1,6-N-Acetylglucosaminidase (dispersin B), which cleaves poly-ss-(1,6)-linked N-acetylglucosamine, is encoded by dspB of Aggregatibacter actinomycetemcomitans. To enhance the production of dispersin B, we engineered dspB to transcribe mRNAs devoid of the trinucleotide ACA. Transcription and translation levels of ACA-less and wild-type dspB expressed in Escherichia coli (E. coli) under T5 and T7 promoters...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
