Article
The discoidin domain of Bacillus circulans β-galactosidase plays an essential role in repressing galactooligosaccharide production.
Bioscience, biotechnology, and biochemistry - 1 Jan 2013
Song Jingyuan, Imanaka Hiroyuki, Imamura Koreyoshi, Minoda Masashi, Yamaguchi Shotaro, Nakanishi Kazuhiro
Abstract excerpt
The recently cloned β-galactosidase from Bacillus circulans ATCC 31382, designated BgaD, contains a multiple domain architecture including a F5/8 type C domain or a discoidin (DS) domain in the C-terminal peptide region. Here we report that the DS domain plays an essential role in repressing the production of galactooligosaccharides (GOSs). We prepared deletion mutants and point-mutated forms of rBgaD-A (deletion...
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