Article
Mutational analysis of the HTLV-I trans-activator, Tax.
FEBS letters - 4 Nov 1991
Heder A, Paca-Uccaralertkun S, Boros I
Abstract excerpt
The gene coding for the trans-activating factor (Tax) of the human T-cell leukemia virus, type I (HTLV-I) was mutagenized in vitro using oligonucleotide-directed mutagenesis and recombinant DNA techniques. All except one of the mutagenized tax constructs failed to trans-activate the HTLV-I LTR in a eukaryotic test system. Moreover, negative Tax mutant Arg-39----Gly was found to be trans-dominant. This observation...
Topics
- Base Sequence
- Blotting, Western
- Chloramphenicol O-Acetyltransferase
- Gene Products, tax
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Plasmids
- Transfection