Article
Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs.
Bioorganic & medicinal chemistry letters - 15 Jul 2009
Simon Grégory, Bouzon Madeleine, Charmantray Franck, Hélaine Virgil, Légeret Bertrand, Marlière Philippe, Hecquet Laurence
Abstract excerpt
Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding alpha-hydroxyaldehydes which could be converted in E....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.