Pantothenate production in Escherichia coli K12 by enhanced expression of the panE gene encoding ketopantoate reductase.

Abstract excerpt

Using gene replacement and transposon Tn5 mutagenesis, an Escherichia coli ilvC panE double mutant completely lacking ketopantoate reductase activity was isolated. This E. coli double mutant was employed to isolate the E. coli panE gene by genetic complementation. The E. coli panE gene is characterized by a 912 bp coding region, which specifies a protein of 303 amino acids with a deduced molecular mass of 33.8...

Topics

• Alcohol Oxidoreductases
• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Genetic Complementation Test
• Molecular Sequence Data
• Mutation
• Pantothenic Acid