Article
Examination of donor substrate conversion in yeast transketolase.
The Journal of biological chemistry - 11 May 2001
Fiedler E, Golbik R, Schneider G, Tittmann K, Neef H, König S, Hübner G
Abstract excerpt
The cleavage of the donor substrate d-xylulose 5-phosphate by wild-type and H263A mutant yeast transketolase was studied using enzyme kinetics and circular dichroism spectroscopy. The enzymes are able to catalyze the cleavage of donor substrates, the first half-reaction, even in the absence of any acceptor substrate yielding d-glyceraldehyde 3-phosphate as measured in the coupled optical test according to...
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