Article
Cloning and characterization of the Bacillus sphaericus genes controlling the bioconversion of pimelate into dethiobiotin.
Gene - 1 Mar 1990
Gloeckler R, Ohsawa I, Speck D, Ledoux C, Bernard S, Zinsius M, Villeval D, Kisou T, Kamogawa K, Lemoine Y
Abstract excerpt
Using 8.8 kb of genetic information from Bacillus sphaericus, it was possible to confer to Escherichia coli bio- strains, including delta bioA-D, bioC-, bioH-, the ability to convert exogenous pimelate into biotin. The bio genes were borne on two recombinant plasmids with inserts of 4.3 kb and 4....
Topics
- Bacillus
- Bacillus subtilis
- Base Sequence
- Biotin
- Biotransformation
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genotype
- Molecular Sequence Data
- Mutation
- Pimelic Acids
- Restriction Mapping