Article
Isolation and characterization of mutants as an approach to a transformation system in Kluyveromyces marxianus.
Current genetics - 1 Jun 1996
Basabe L, Cabrera N, Yong V, Menéndez J, Delgado J M, Rodríguez L
Abstract excerpt
A method to obtain K. marxianus mutants has been developed. Different auxotrophic mutants were isolated by nystatin and snail-enzyme enrichment procedures using an incubation time of 2 h before adding the antibiotic or the enzyme respectively. All his mutants analyzed by complementation tests tur...
Topics
- Genes, Fungal
- Genetic Complementation Test
- Histidine
- Kluyveromyces
- Mutagenesis
- Mutation
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae
- Transformation, Genetic