Article
Isolation and characterization of mutants as an approach to a transformation system in Kluyveromyces marxianus.
Current genetics - 1 Jun 1996
Basabe L, Cabrera N, Yong V, Menéndez J, Delgado J M, Rodríguez L
Abstract excerpt
A method to obtain K. marxianus mutants has been developed. Different auxotrophic mutants were isolated by nystatin and snail-enzyme enrichment procedures using an incubation time of 2 h before adding the antibiotic or the enzyme respectively. All his mutants analyzed by complementation tests turned out to belong to the same complementation group. Some of them were transformed and complemented by the S....
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