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Article

Identification and quantification of glycoproteins using ion-pairing normal-phase liquid chromatography and mass spectrometry.

Molecular & cellular proteomics : MCP - 1 Sept 2009

Ding Wen, Nothaft Harald, Szymanski Christine M, Kelly John

Abstract excerpt

Glycoprotein structure determination and quantification by MS requires efficient isolation of glycopeptides from a proteolytic digest of complex protein mixtures. Here we describe that the use of acids as ion-pairing reagents in normal-phase chromatography (IP-NPLC) considerably increases the hydrophobicity differences between non-glycopeptides and glycopeptides, thereby resulting in the reproducible isolation of...

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Identifiers

PMID: 19525481
DOI: 10.1074/mcp.M900088-MCP200
PMCID: PMC2742440

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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