Article
Affinity-capture tandem mass spectrometric characterization of polyprenyl-linked oligosaccharides: tool to study protein N-glycosylation pathways.
Analytical chemistry - 15 Jul 2008
Reid Christopher W, Stupak Jacek, Chen Mark M, Imperiali Barbara, Li Jianjun, Szymanski Christine M
Abstract excerpt
N-glycosylation of proteins is recognized as one of the most common post-translational modifications. Until recently it was believed that N-glycosylation occurred exclusively in eukaryotes before the discovery of the general protein glycosylation pathway (Pgl) in Campylobacter jejuni. To date, most techniques to analyze lipid-linked oligosaccharides (LLOs) of these pathways involve the use of radiolabels and...
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