Article
High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS.
Analytical chemistry - 21 Jan 2020
Techner José-Marc, Kightlinger Weston, Lin Liang, Hershewe Jasmine, Ramesh Ashvita, DeLisa Matthew P, Jewett Michael C, Mrksich Milan
Abstract excerpt
High-throughput quantification of the post-translational modification of many individual protein samples is challenging with current label-based methods. This paper demonstrates an efficient method that addresses this gap by combining Escherichia coli-based cell-free protein synthesis (CFPS) and self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry (SAMDI-MS) to analyze...
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