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Article

Determination of DNA mutation load in human tissues using denaturing HPLC-based heteroduplex analysis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Lim Kok Seong, Naviaux Robert K, Haas Richard H

Abstract excerpt

Since its introduction more than a decade ago, denaturing HPLC has been widely used to screen nuclear and mitochondrial DNA for mutations. We recently developed a quantitative method based on denaturing HPLC to measure DNA mutation load, using tRNA Leu(UUR) region of the mitochondrial DNA as an example. The mutation load is determined based on the quadratic function that governs DNA reannealing and the formation...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 19513681
DOI: 10.1007/978-1-59745-521-3_18

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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