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Article

Pitfalls in the denaturing high-performance liquid chromatography analysis of mitochondrial DNA mutation.

The Journal of molecular diagnostics : JMD - 1 Jan 2008

Lim Kok Seong, Naviaux Robert K, Wong Scott, Haas Richard H

Abstract excerpt

Denaturing high-performance liquid chromatography (DHPLC) purification of heteroduplexes has been reported as a method to increase sensitivity of the detection of low-level heteroplasmy by DNA sequencing, and DHPLC profiling has been suggested as a method to allow the correlation of a characteristic chromatographic profile with a specific sequence alteration. Herein we report pitfalls associated with the use of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Mitochondrial

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Share to RNA, Transfer, Leu

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Identifiers

PMID: 18165269
DOI: 10.2353/jmoldx.2008.070081
PMCID: PMC2175549

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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