Article
Clinical applications of denaturing high-performance liquid chromatography-based genotyping.
Croatian medical journal - 1 Aug 2003
Danielson Phillip B, Shelton Robert J, LaBerge Greggory S
Abstract excerpt
AIM: To develop and evaluate heteroduplex forming templates (HFTs) as a common set of molecular standards for genotyping by denaturing high-performance liquid chromatography (DHPLC) using hypervariable regions of human mitochondrial DNA (mtDNA) as a model system. METHODS: Hypervariable regions 1 and 2 from the mtDNA D-loop of 22 maternally related and unrelated human volunteers were amplified by polymerase chain...
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