Article
Knock-in and knock-out: the use of reverse genetics in somatic cells to dissect mitotic pathways.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Barr Alexis R, Zyss Deborah, Gergely Fanni
Abstract excerpt
Reverse genetic methods, such as homologous gene targeting, have greatly contributed to our understanding of molecular pathways in mitosis, especially in yeast. The chicken B-lymphocyte line, DT40, represents a unique example among vertebrate somatic cells where homologous gene targeting occurs at very high frequency. DT40 cells therefore provide a useful and accessible somatic genetic system for wide-ranging...
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