Article
In vivo multiplex quantitative analysis of 3 forms of alpha melanocyte stimulating hormone in pituitary of prolyl endopeptidase deficient mice.
Molecular brain - 2 Jun 2009
Perroud Bertrand, Alvarado Rudy J, Espinal Glenda M, Morado Alex R, Phinney Brett S, Warden Craig H
Abstract excerpt
BACKGROUND: In vitro reactions are useful to identify putative enzyme substrates, but in vivo validation is required to identify actual enzyme substrates that have biological meaning. To investigate in vivo effects of prolyl endopeptidase (PREP), a serine protease, on alpha melanocyte stimulating hormone (alpha-MSH), we developed a new mass spectrometry based technique to quantitate, in multiplex, the various...
Topics
- Acetylation
- Animals
- Blotting, Western
- Genotype
- Mice
- Pituitary Gland
- Prolyl Oligopeptidases
- Protein Isoforms
- Proteomics
- Serine Endopeptidases
- Substrate Specificity
- Time Factors
- alpha-MSH