Article
Biosynthesis and posttranslational processing of site-directed endoproteolytic cleavage mutants of pro-neuropeptide Y in mouse pituitary cells.
The Journal of biological chemistry - 15 Feb 1990
Dickerson I M, Dixon J E, Mains R E
Abstract excerpt
Although pairs of basic amino acids are common endoproteolytic sites in prohormones, the enzymes responsible for these cleavages have not yet been characterized. To investigate the specificity of these endoproteases, cDNAs encoding pro-neuropeptide Y (pro-NPY) containing all four pairs of basic a...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- DNA Probes
- Humans
- Kinetics
- Mice
- Molecular Sequence Data
- Mutation
- Neuropeptide Y
- Peptide Mapping
- Pituitary Gland
- Plasmids
- Protein Precursors
- Protein Processing, Post-Translational