Article
Multiple genetic manipulations of DT40 cell line.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Motegi Akira, Takata Minoru
Abstract excerpt
Reverse genetics is gaining importance in the field of modern biological sciences. Gene disruption and the use of siRNAs are the favored techniques for current research. Many researchers, however, are aware that the data from siRNA experiments are frequently inconsistent and that epistatic analysis of multiple genes using siRNAs is barely feasible. In recognition of the drawbacks of the siRNA technique, many...
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