Article
Using DNAWorks in designing oligonucleotides for PCR-based gene synthesis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Hoover David
Abstract excerpt
The availability of sequences of entire genomes has dramatically increased the number of protein targets, many of which will need to be overexpressed in cells other than where they have been identified originally. Gene synthesis often provides a fast and economically efficient approach. The synthetic gene can be optimized for expression and constructed for easy mutational manipulation without regard for the...
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